FuGENE HD Transfection Reagent from Promega

Product Specs
ItemFuGENE HD Transfection Reagent
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE2311
Quantity1ml
Molecular Weight~86kDa
Transfection MethodNon-liposomal
Transfection Sample TypeDNA, and Plasmid

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) A farnesyl-dependent structural role for CENP-E in expansion of the fibrous coronaJournal of Cell BiologyJanuary 1, 2024Jingchao Wu, Maximilian W.D. Raas, Paula Sobrevals Alcaraz, Harmjan R. Vos, Eelco C. Tromer, Berend Snel, Geert J.P.L. Kopsvesicular stomatitis virus G glycoprotein, Rev, and Gag-Pol, with the transfection reagent of Fugene HD (E2311; Promega). 72 h after transfection, cell culture supernatant was harvested and filtered. Stable cell

(2) FAT10 is phosphorylated by IKKβ to inhibit the antiviral type-I interferon responseLife Science AllianceJanuary 1, 2024Kritika Saxena, Nicola Domenico Roverato, Melody Reithmann, Mei Min Mah, Richard Schregle, Gunter Schmidtke, Ivan Silbern, Henning Urlaub, Annette Aichemtransfection reagent (408727; Merck), and for transfection of A549 cells, FuGENE transfection reagent (E2311; Promega) was used. In case of a 100-mm2 cell culture dish, 600 μl serum and antibiotic-free medium

(3) miR136 regulates proliferation and differentiation of small tail han sheep preadipocytesAdipocyteDecember 31, 2023Man Luo, Lin Wang, Cheng Xiao, Mengsi Zhou, Minghui Li, Hongjuan LiµL) were respectively transfected to the cells with FuGene HD Transfection Reagent (10 µL) (Promega, E2311) according to the manufacturer’s instructions. RNA interference The small-interfering RNA (siRNA)

(4) Voltage sensors of a Na+ channel dissociate from the pore domain and form inter-channel dimers in the resting stateNat CommunDecember 19, 2023Ayumi Sumino, Takashi Sumikama, Mikihiro Shibata, Katsumasa Iriegreen fluorescent protein (EGFP)-cloned pBiEX vectors using FuGENE HD transfection reagent (Promega; E2311). First, the channel-cloned vector (1.5 μg) was mixed with 0.5 μg of the EGFP-cloned vector in 100 μL

(5) CiRS-7 Enhances the Liquid-liquid Phase Separation of miRISC and Promotes DNA Damage RepairNucleusDecember 18, 2023Yun-Long Wang, Li-Li Feng, Jie Shi, Wan-Ying Chen, Shu-Ying Bie, Shao-Mei Bai, Guang-Dong Zeng, Rui-Zhi Wang, Jian Zheng, Xiang-Bo Wan, et al.Lipofectamine 2000 (11668019, Invitrogen, ThermoFisher Scientific) or FuGENE HD Transfection Reagent (E2311, Promega, Madison, Wisconsin, USA), while transfections of siRNA or miRNA mimics were conducted with

(6) ERK2-topoisomerase II regulatory axis is important for gene activation in immediate early genesNat CommunDecember 14, 2023Heeyoun Bunch, Deukyeong Kim, Masahiro Naganuma, Reiko Nakagawa, Anh Cong, Jaehyeon Jeong, Haruhiko Ehara, Hongha Vu, Jeong Ho Chang, Matthew J. Schellenberg, et al.(Santa Cruz Biotechnology, sc-35335), or both ERK siRNA species. Lipofectamine 2000 (Invitrogen) or FuGENE HD (Promega, E2311) transfection reagent was used according to the manufacturer’s instructions. The transfected cells were

(7) Faecalibacterium prausnitzii promotes intestinal epithelial IL-18 production through activation of the HIF1α pathwayFront MicrobiolDecember 14, 2023Raphael R. Fagundes, Gabriela Bravo-Ruiseco, Shixian Hu, Sarah J. Kierans, Rinse K. Weersma, Cormac T. Taylor, Gerard Dijkstra, Hermie J. M. Harmsen, Klaas Nico Faber71409). For lentivirus production, HEK293T cells in T25 were transfected using 15 μL Fugene HD (Promega, E2311), 250 μL Opti-MEM, 1.5 μg Lenti-iCas9-neo (Addgene, 85400) or pLenti-sgRNA (see above), 1 μg pMD2.G

(8) Paradoxical dominant negative activity of an immunodeficiency-associated activating PIK3R1 variantbioRxivDecember 13, 2023Patsy R. Tomlinson, Rachel Knox, Olga Perisic, Helen C. Su, Gemma V. Brierley, Roger L. Williams, Robert K. Semple(Thermo Scientific). 2–4μg bacmid was transfected into Sf9 cells in 6 well plates using Insect-XPRESS (Promega E2311). Cells were incubated at 2°C for 5 days and bacmid YFP expression assessed by Leica DM IL LED Fluo

(9) Uridine–cytidine kinase 2 potentiates the mutagenic influence of the antiviral β-d-N4-hydroxycytidineNucleic Acids ResearchDecember 11, 2023Zhen Xu, Christoffer Flensburg, Rebecca A Bilardi, Ian J Majewski(Addgene 8454)) for each 10 cm dish of HEK293T using 24 μl FuGENE HD Transfection Reagent (Promega, E2311). Ten dishes of HEK293T cells were used to achieve a good representation of each sgRNA. Supernatants

(10) A chromosome-level genome assembly for the Silkie chicken resolves complete sequences for key chicken metabolic, reproductive, and immunity genesCommun BiolDecember 6, 2023Feng Zhu, Zhong-Tao Yin, Qiang-Sen Zhao, Yun-Xiao Sun, Yu-Chen Jie, Jacqueline Smith, Yu-Ze Yang, David W. Burt, Maxwell Hincke, Zi-Ding Zhang, et al.codon usage and reduce the GC content. Plasmids were transfected into cells by FuGENE reagent (Promega #E2311). Leptin coding sequence verification As the full-length leptin failed to be amplified with alternative

(11) A non-conducting role of the Ca v 1.4 Ca 2+ channel drives homeostatic plasticity at the cone photoreceptor synapsebioRxivDecember 6, 2023J. Wesley Maddox, Gregory J. Ordemann, Juan de la Rosa Vázquez, Angie Huang, Christof Gault, Serena R. Wisner, Kate Randall, Daiki Futagi, Steven H. DeVries, Mrinalini Hoon, et al.(EGTA)",Sigma-Aldrich,03777 Fetal Bovine Serum (FBS),Gibco,16140–071 FuGENE HD Transfection reagent,Promega,E2311 Guanosine 5’-triphosphate sodium salt hydrate,Sigma-Aldrich,G8877 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic

(12) Tetherin antagonism by SARS‐CoV‐2 ORF3a and spike protein enhances virus releaseEMBO reportsDecember 6, 2023Hazel Stewart, Roberta Palmulli, Kristoffer H Johansen, Naomi McGovern, Ola M Shehata, George W Carnell, Hannah K Jackson, Jin S Lee, Jonathan C Brown, Thomas Burgoyne, et al.HEK‐293T cells were transfected with 1 μg of plasmid DNA complexed to 3 μl of FuGENE HD (Promega, Cat: E2311). 16–20 h post transfection the cells were dissociated from the plastic using cell dissociation buffer

(13) Systematic assessment of transcriptomic and metabolic reprogramming by blue light exposure coupled with agingPNAS NexusDecember 5, 2023Jia Huang, Fan Zhou, Huanchan Zhou, Xiaoqi Zheng, Zhengyi Huo, Meng Yang, Zihe Xu, Runzhou Liu, Luoluo Wang, Xiaoyun WangA total of 20 μg dsRNA targeting Mettl3, fl(2)d, or Tor and 10 μL FuGENE HD Transfection Reagent (#E2311, Promega) were added to 100 μL serum-supplemented medium containing cultured S2 cells at a density of

(14) Epicardioid single-cell genomics uncovers principles of human epicardium biology in heart development and diseaseNature biotechnologyDecember 1, 2023Meier AB, Zawada D, De Angelis MT, Martens LD, Santamaria G, Zengerle S, Nowak-Imialek M, Kornherr J, Zhang F, Tian Q, et al.transfer vector, the CMVDR8.74 packaging plasmid and the VGV.G envelope plasmid using Fugene HD (Promega, E2311). Viral supernatants were collected after 48 h and used for infection of epicardioids derived from the

(15) Functional assessment of lysosomal Rab7 and RILP with RNA interference and overexpression in Spodoptera frugiperda Sf9 cell linesSTAR ProtocolsDecember 1, 2023Gaofeng Cui, Zhiyan Jiang, Guohua ZhongT7 RiboMAX Express RNAi System,"Promega, USA",Cat#P1700 FuGENE HD transfection reagent,"Promega, USA",Cat#E2311 E.Z.N.A. Endo-Free Plasmid Mini Kit II,"Omega, USA",Cat#D6950-01 Universal DNA Purification Kit,"TIANGEN,

(16) Quantification of ligand and mutation-induced bias in EGFR phosphorylation in direct response to ligand bindingNature CommunicationsNovember 21, 2023Daniel Wirth, Ece Özdemir, Kalina Hristovawell. 24 h later, the cells were transfected with 1 or 1.5 µg plasmid DNA using FuGene HD (Promega, #E2311) according to the manufacturer’s protocol. 36 h after transfection, vesiculation was induced using osmotic

(17) The fusion gene hsf5-rnf43 in Nile tilapia: A potential regulator in the maintenance of testis function and sexual differentiationiScienceNovember 17, 2023Yawei Shen, Hewei Jiang, Adelino V.M. Canario, Tiantian Chen, Yufei Liu, Guokun Yang, Xiaolin Meng, Jinliang Zhao, Xiaowu ChenA600701 Eosin,Sangon Biotech,Order No. E607321 RNAlater™,Invitrogen,Cat. No. 4427575 FuGENE® HD,Promega,Cat. No. E2311 Bgl II,Takara,Code No. 1021A Hind III,Takara,Code No. 1060S ,, Critical commercial assays,Critical

(18) RACK1 promotes Shigella flexneri actin-mediated invasion, motility, and cell-to-cell spreadingiScienceNovember 17, 2023Karla N. Valenzuela-Valderas, Elmira Farrashzadeh, Yuen-Yan Chang, Yunnuo Shi, Renee Raudonis, Brendan M. Leung, John R. Rohde, Jost Enninga, Zhenyu ChengA11139-03 Gentamycin,Sigma,Cat. No. G4918 Polyethyleneimine (PEI),Sigma,Cat. No. 765090 FuGENE,Promega,Cat. No. E2311 X-tremeGENE,Sigma,Cat. No. 6365779001 FluoroBrite DMEM,Gibco,Cat. No. A1896701 Fibronectin,Sigma,Cat.

(19) Compartmentalization of androgen receptors at endogenous genes in living cellsNucleic Acids ResearchNovember 10, 2023Selçuk Yavuz, Hélène Kabbech, Jente van Staalduinen, Simon Linder, Wiggert A van Cappellen, Alex L Nigg, Tsion E Abraham, Johan A Slotman, Marti Quevedo, Raymond A Poot, et al.the PX459-sgRNA plasmid and a repair template were transfected for each gene using Fugene 6 (Promega: E2311) according to the manufacturer's protocol. Cells were pre-seeded on a 6-well plate and transfected the

(20) Cytoplasmic-delivery of polyinosine-polycytidylic acid inhibits pancreatic cancer progression increasing survival by activating Stat1-CCL2-mediated immunityJournal for ImmunoTherapy of CancerNovember 7, 2023Praveen Bhoopathi, Amit Kumar, Anjan K Pradhan, Santanu Maji, Padmanabhan Mannangatti, Jolene J Windle, Mark A Subler, Dongyu Zhang, Vignesh Vudatha, Jose G Trevino, et al.12 Plasmid transfections Plasmid transfection experiments used FuGene HD transfection reagent (Cat. No E2311, Promega, Madison, Wisconsin, USA) using the manufacturer’s protocol. Details provided in online supplemental

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(1)

5/5/2021Efficient in Transfecting Plasmid DNA into HEK293 cells Niroj Shrestha0.5 µg of ER-localized mEmerald-ER3 DNA and 0.75 µl fugene HD were diluted in 200 µl Optimem and incubated for 15 min. The complex was added to the culture medium with HEK293 cells (around 50% ... Read Review
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11/4/2015Transfection Using Fugene HD Kawalpreet AnejaThis product was used for transfection of JEG3 cell lines.